Real-Time PCR test in diagnosis of certain sexually transmitted diseases

Article by Doctor of Laboratory Department – Share99 Danang International Health Hub

Real-Time PCR (Polymerase Chain Reaction) testing techniques are used to amplify and at the same time determine the number of target DNA molecules. Realtime PCR testing is useful for accurately identifying and describing pathogens that play an important role in the treatment of sexually transmitted diseases.

1. Diagnostic tests for sexually transmitted diseases:

With sexually transmitted diseases, testing helps to identify specific causes of each different pathology, from which the doctor can come up with a method suitable for each cause of the disease as well as measures to prevent re-infection and minimize the risk of infection. The detection of pathogens in pregnant women also allows to manage the disease, avoiding transmission of the disease to the child at birth.

However, many types of infection cannot be traced to the immediate or asymptoatic cause, so it is necessary to use identification systems that give fast and accurate results. The Real-Time PCR technique can measure the number of target DNA molecules present in the sample in the reaction's amplification cycle. With a sensitivity and specificity of over 98% in the detection of dnas of pathogens, the above detected DNA concentrations for pathogens. Therefore, Realtime PCR techniques become useful for identifying and accurately describing pathogens that play a role in the treatment of STIs.

PCR Testing

Real-Time PCR Technique

The advantage of the realtime PCR technique is the detection of amplification segments based on the use of fluorescent primers attached to the DNA double helix generated during the thermal cycle. Fluorescent signals are recorded in real time, eliminating the risk of infection due to post-PCR manipulations.

REALQUALITY RQ-SevenSTI kit, code RQ-127, AB ANALITICA (Italy), using Real-Time amplification techniques (Real-Time PCR) to detect 7 common agents of sexually transmitted diseases: Chlamydiatrachomatis, Neisseriagonorrhoeae, Mycoplasmahominis, Mycoplasmagenitalium, Trichomonasvaginalis, Ureaplasma parvum and Ureaplasmaurealyticum

The kit enables to control the extraction process and assess the presence of reaction inhibitors in the sample by amplifying the β-globin gene in multiplex reaction with the target agent, avoiding false negatives as well as providing reaction to help fight off-infection from the previous amplification

RT-PCR is used to diagnose the virus that causes the disease

Bộ kit Real-Time (Real-Time PCR)

2. Collect samples

2.1 Considerations for performing a diagnosis test for sexually transmitted diseases

  • Describe the full symptoms that you have with your doctor during your visit.
  • Do not rinse your genitals or have intercourse for at least 24 hours.
  • For women who are in the menstrual cycle or have vaginal bleeding, this test must not be done

2.2. Specify

  • Subjects: People with suspected symptoms of sexually transmitted diseases such as urinary tract, urination, abnormal vaginal/urethra discharge, itching/burning, genital ulcers, smoldering pelvic pain. Especially in women who are pregnant in the first 3 months
  • High-risk subjects: People who have uns secure sex, multiple sexual partners, partners with suspected STI symptoms.
  • Have another cause of STI.

2.3 How to take samples and preserve

Specimens: commonly used in the diagnosis of sexually transmitted diseases including

Cell specimens:

  • Samples of cervical, vaginal, vaginal/urethral smear samples, rectal and semen smear samples using small swabs or eptic brooms, contained in transportable ileum vials (PBS, biological/isopic solution or other environment)

Urine samples

  • The detection of sexually transmitted infections is carried out with early morning urine samples. Urine samples must be collected in erile containers,

Mid-line urine

Urine samples used as tests


  • Samples should be transported early to the Laboratory to avoid DNA degeneration, misleading the results.
  • Store samples at +2 °C to +8 °C and extract nucleic acids within 48 hours.
  • If extraction cannot be separated within 48 hours, store the sample at -30°C to -20°C

Share99 International Health Hub provides a Package of Examination and Screening for social diseases so that customers can detect diseases early and have an effective treatment direction, preventing dangerous complications. The social disease screening package at Share99 is open to all ages, both men and women.

To register for examination and treatment at Share99 International Health Hub, you can contact Share99 Health System nationwide, or register for an online examination HERE.


  • Guidance on diagnosis and treatment of sexually transmitted diseases (2013), Ministry of Health.
  • Sexually Transmitted Diseases Treatment Guidelines (2015), U.S. Department of Health and Human Services, Centers for Disease Control and Prevention.


  • Common sexually transmitted diseases today
  • What is a PCR test?
  • Learn PCR tests for gonorrhea and chlamydia


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  • Is chlamydia dangerous?
  • Learn PCR tests for gonorrhea and chlamydia
  • Spoiled gas and white blood: What to know

About: Minh Quynh

b1ffdb54307529964874ff53a5c5de33?s=90&d=identicon&r=gI am the author of I had been working in Vinmec International General Hospital for over 10 years. I dedicate my passion on every post in this site.


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